{"id":12983,"date":"2023-06-30T13:12:07","date_gmt":"2023-06-30T05:12:07","guid":{"rendered":"https:\/\/lfz100.ust.hk\/wordpress\/?page_id=12983"},"modified":"2023-06-30T15:21:53","modified_gmt":"2023-06-30T07:21:53","slug":"cut-tag","status":"publish","type":"page","link":"https:\/\/lfz100.ust.hk\/wordpress\/cut-tag\/","title":{"rendered":"Cut &#038; Tag"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"12983\" class=\"elementor elementor-12983\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3518326d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3518326d\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-66 elementor-top-column elementor-element elementor-element-68f92b0a\" data-id=\"68f92b0a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3a156fb3 elementor-widget elementor-widget-spacer\" data-id=\"3a156fb3\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.6.0 - 21-03-2022 *\/\n.e-container.e-container--row .elementor-spacer-inner{width:var(--spacer-size)}.e-container.e-container--column .elementor-spacer-inner,.elementor-column .elementor-spacer-inner{height:var(--spacer-size)}<\/style>\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-41125512 elementor-widget elementor-widget-image\" data-id=\"41125512\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.6.0 - 21-03-2022 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"624\" height=\"778\" src=\"https:\/\/lfz100.ust.hk\/wordpress\/wp-content\/uploads\/2023\/06\/Picture2.png\" class=\"attachment-medium_large size-medium_large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/lfz100.ust.hk\/wordpress\/wp-content\/uploads\/2023\/06\/Picture2.png 624w, https:\/\/lfz100.ust.hk\/wordpress\/wp-content\/uploads\/2023\/06\/Picture2-241x300.png 241w\" sizes=\"(max-width: 624px) 100vw, 624px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-11edc76b elementor-widget elementor-widget-text-editor\" data-id=\"11edc76b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.6.0 - 21-03-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>\u00a0* Image from activemotif.com\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-237f0758\" data-id=\"237f0758\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7a5948e4 elementor-widget elementor-widget-spacer\" data-id=\"7a5948e4\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-64563b79 elementor-widget elementor-widget-heading\" data-id=\"64563b79\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.6.0 - 21-03-2022 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h1 class=\"elementor-heading-title elementor-size-default\">CUT&amp;Tag <br><\/h1>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4db94ce0 elementor-widget elementor-widget-text-editor\" data-id=\"4db94ce0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"color: #050e17; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">CUT&amp;Tag (Cleavage Under Targets and Tagmentation) is a\u00a0<\/span><a class=\"MarkdownLink_linkifiedLink__KxC9G\" style=\"cursor: pointer; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">molecular biology<\/a><span style=\"color: #050e17; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">\u00a0method used to investigate protein-DNA interactions and identify\u00a0<\/span><a class=\"MarkdownLink_linkifiedLink__KxC9G\" style=\"cursor: pointer; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">DNA binding sites<\/a><span style=\"color: #050e17; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">\u00a0for a protein of interest. Similar to\u00a0<\/span><a class=\"MarkdownLink_linkifiedLink__KxC9G\" style=\"cursor: pointer; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">ChIP<\/a><span style=\"color: #050e17; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">, CUT&amp;Tag uses antibodies to bind to the target protein, but instead of an\u00a0<\/span><a class=\"MarkdownLink_linkifiedLink__KxC9G\" style=\"cursor: pointer; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">immunoprecipitation step<\/a><span style=\"color: #050e17; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">, the chromatin is sheared and\u00a0<\/span><a class=\"MarkdownLink_linkifiedLink__KxC9G\" style=\"cursor: pointer; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">library preparation<\/a><span style=\"color: #050e17; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">\u00a0is performed using a\u00a0<\/span><a class=\"MarkdownLink_linkifiedLink__KxC9G\" style=\"cursor: pointer; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">Tn5 transposase<\/a><span style=\"color: #050e17; font-family: -apple-system, system-ui, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 18px; background-color: #f1f2f2;\">\u00a0fused with protein A to direct the enzyme to the antibody-bound target on chromatin. CUT&amp;Tag has several advantages over ChIP, including its sensitivity and ability to generate robust data with lower background signal. Additionally, it has potential for single-cell experiments.<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-746bfa21 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"746bfa21\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a href=\"https:\/\/lfz100.ust.hk\/wordpress\/wp-content\/uploads\/2023\/06\/cut-and-tag-protocol.docx\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-lg elementor-animation-grow\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-text\">Protocol<\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\u00a0* Image from activemotif.com\u00a0 CUT&amp;Tag CUT&amp;Tag (Cleavage Under Targets and Tagmentation) is a\u00a0molecular biology\u00a0method used to investigate protein-DNA interactions and identify\u00a0DNA binding sites\u00a0for a protein of interest. Similar to\u00a0ChIP, CUT&amp;Tag &#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/pages\/12983"}],"collection":[{"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/comments?post=12983"}],"version-history":[{"count":19,"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/pages\/12983\/revisions"}],"predecessor-version":[{"id":13008,"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/pages\/12983\/revisions\/13008"}],"wp:attachment":[{"href":"https:\/\/lfz100.ust.hk\/wordpress\/wp-json\/wp\/v2\/media?parent=12983"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}