Next-generation sequencing (NGS) is a high-throughput, massively parallel technology which allows rapid, large-scale sequencing. A fundamental step for NGS is the library construction of the samples of interest so that they are compatible to a sequencer. First, DNA fragments are end repaired and dA-tailed to eliminate overhangs and to prevent downstream concatemer ligation.  Then platform-specific adaptor oligos are ligated to the fragments to allow binding to the flow cells.  Samples are amplified if necessary.

The protocol below is for the Illumina platform.