Whole-genome bisulfite sequencing (WGBS) is a molecular technique that combines sodium bisulfite treatment with high-throughput sequencing methods to interrogate the genome-wide state of DNA methylation.  Methylation at the fifth position in cytosine nucleotides (5-mC) is an epigenetic modification, integral to gene regulation and other transcriptional activities.  Sheared genomic DNA is prepped for sequencing through library construction and followed by bisulfite conversion in which unmethylated cytosine will be converted into uracil while methylated cytosine will remain unaffected.

The protocol below uses the KAPA library preparation kit before bisulfite treatment.