ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique for assessing genome-wide chromatin accessibility. This method utilizes hyperactive Tn5 transposase to simultaneously cut and incorporate sequencing adapters into regions of open chromatin.  Mapped sequencing reads can be used to infer regions of increased accessibility, nucleosome positioning and transcription factor binding sites.  

There are 2 protocols below: “In-House” uses Tn5 generated in the lab while “Kit” uses commercial Tn5.