CUT&Tag

CUT&Tag (Cleavage Under Targets and Tagmentation) is a molecular biology method used to investigate protein-DNA interactions and identify DNA binding sites for a protein of interest. Similar to ChIP, CUT&Tag uses antibodies to bind to the target protein, but instead of an immunoprecipitation step, the chromatin is sheared and library preparation is performed using a Tn5 transposase fused with protein A to direct the enzyme to the antibody-bound target on chromatin. CUT&Tag has several advantages over ChIP, including its sensitivity and ability to generate robust data with lower background signal. Additionally, it has potential for single-cell experiments.